Microbial Process Indicators in Activated Sludge Process

Following taxonomic identification, enumeration and evaluation of the characteristics of the various organisms and structures present in a wastewater sample, the information can be used to draw conclusions regarding the treatment process.

Dispersed growth is material suspended within the activated sludge process that has not been adsorbed into the floc particles. This material consists of very small quantities of colloidal (too small to settle out) bacteria as well as organic and inorganic particulate material. While a small amount of dispersed growth in between the floc particles is normal, excessive amounts can be carried through a secondary clarifier. When discharged from the treatment plant, dispersed growth results in higher effluent solids.

Within most activated sludge processes, the shape of the floc particles can indicate certain environmental or operational conditions. A spherical floc particle indicates immature floc, as would be found during start-up or a process recovery. A mature floc particle of irregular shape indicates the presence of a beneficial quantity of filamentous organisms and good quality effluent.

An excess of dispersed growth could indicate a very young sludge, the presence of toxic material, excess mechanical aeration or an extended period of time at low dissolved oxygen levels. Certain protozoans, such as amoebae and flagellates dominate during a system start-up. Free swimming ciliates are indicative of a sludge of intermediate health and an effluent of acceptable or satisfactory quality. A predominance of crawling ciliates, stalked ciliates and metazoans is an indicator of sludge with excellent health and an effluent of high quality.

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